Analog isomer bicyclo[4.1.0]heptana saka inhibitor glikosidase galacto-validamine, (1R*,2S,3S,4S,5S,6S*)-5-amino-1-(hydroxymethyl)bicyclo[4.1.0]heptane -2,3,4-triol, wis disintesis ing 13 langkah saka 2,3,4,6-tetra-O-benzyl-D-galaktosa.Aktivitas hambat saka loro amina sing diwatesi sacara konformasi, lan asetamida sing cocog, diukur nglawan enzim alpha-galactosidase komersial saka biji kopi lan E. coli.Aktivitas enzim GH27 kulawarga glikosil hidrolase (biji kopi) dicegah kanthi kompetitif dening 1R,6S-amin (7), interaksi ikatan sing ditondoi kanthi nilai K(i) 0,541 mikroM.GH36 E. coli alpha-galactosidase nuduhake interaksi ikatan sing luwih lemah karo 1R,6S-amine (IC(50)= 80 microM).Diastereomeric 1S,6R-amine (9) kaiket lemah karo loro galaktosidase, (biji kopi, IC(50)= 286 microM) lan (E. coli, IC(50)= 2,46 mM).
