dCMP, 2′-Deoxycytidine 5′-monophosphate, asam bebas CAS:1032-65-1
| Nomer Katalog | XD90549 |
| Jeneng produk | dCMP, 2'-Deoxycytidine 5'-monophosphate, asam bebas |
| CAS | 1032-65-1 |
| Formula Molekul | C9H14N3O7P |
| Bobot Molekul | 307.20 |
| Rincian panyimpenan | -15 nganti -20 °C |
| Kode Tarif Harmonisasi | 29349990 |
Spesifikasi Produk
| Penampilan | Padat |
| Assay | 99% |
Beta-D-2'-deoxy-2'-fluoro-2'-C-methylcytidine (PSI-6130) minangka inhibitor spesifik kuat sintesis RNA virus hepatitis C (HCV) ing sel replika Huh-7.Kanggo nyandhet polimerase RNA HCV NS5B, PSI-6130 kudu difosforilasi dadi bentuk 5'-trifosfat.Fosforilasi PSI-6130 lan inhibisi HCV NS5B diselidiki.Fosforilasi PSI-6130 dening rekombinan manungsa 2'-deoxycytidine kinase (dCK) lan uridine-cytidine kinase 1 (UCK-1) diukur kanthi nggunakake reaksi spektrofotometri gabungan.PSI-6130 dituduhake minangka substrat kanggo dCK sing diresiki, kanthi Km 81 microM lan kcat 0,007 s-1, nanging ora dadi substrat kanggo UCK-1.PSI-6130 monophosphate (PSI-6130-MP) difosforilasi kanthi efisien dadi difosfat lan banjur dadi trifosfat kanthi rekombinan manungsa UMP-CMP kinase lan nukleosida difosfat kinase.Inhibisi polimerase RNA tipe liar lan mutasi (S282T) HCV NS5B RNA diteliti.Konstanta inhibisi steady-state (Ki) kanggo PSI-61 30 trifosfat (PSI-6130-TP) kanthi enzim jinis liar yaiku 4,3 microM.Asil sing padha ditampa karo 2'-C-methyladenosine triphosphate (Ki=1,5 microM) lan 2'-C-methylcytidine triphosphate (Ki=1,6 microM).NS5B kanthi mutasi S282T, sing dikenal menehi resistensi marang 2'-C-methyladenosine, dicegah dening PSI-6130-TP kanthi efisien kaya jinis liar.Penggabungan PSI-6130-MP menyang RNA sing dikatalisis dening polimerase RNA NS5B sing dimurnèkaké nyebabake putus rantai.
