γ-L-Glutamyl-3-carboxy-4-nitroanilide, garam monoamonium Cas:63699-78-5 Serbuk mikrokristalin kuning muda
| Nomer Katalog | XD90183 |
| Jeneng produk | γ-L-Glutamyl-3-carboxy-4-nitroanilide, uyah monoamonium |
| CAS | 63699-78-5 |
| Formula Molekul | C12H12N3O7-.H4N+ |
| Bobot Molekul | 328.27 |
| Rincian panyimpenan | Ambient |
Spesifikasi Produk
| Penampilan | Bubuk microcrystalline kuning cahya |
| Assay | 99% |
| Titik lebur | 187ºC ± 2.0ºC |
| Titik didih | 718,3 ° C ing 760 mmHg |
| Titik nyala | 368,5 °C |
| Kelarutan | H2O: 100 mg/mL, bening, kuning-ijo |
Kinetika serum manungsa gamma-glutamyltransferase (EC 2.3.2.2) diselidiki, kanthi nggunakake glycylglycine minangka substrat akseptor gamma-glutamyl lan gamma-glutamyl-4-nitroanilide lan turunan karboksi, gamma-glutamyl-3-carboxy-4 -nitroanilide, minangka substrat donor.Kedadeyan simultan saka gamma-glutamyltransfer lan autotransfer ditetepake kanthi kromatografi kertas mudhun.Plot timbal balik ganda rasio konstan ngonfirmasi yen mekanisme enzim ora urutan (ping-pong bi-bi).Inhibisi dening salah siji donor ora ditemokake, lan inhibisi dening glycylglycine mung diamati ing konsentrasi ing ndhuwur kapentingan klinis.Konstanta kinetik sing dipikolehi kanthi analisis regresi nonlinier saka data kecepatan awal digunakake kanggo nemtokake konsentrasi substrat reagen kanggo uji enzim iki.Pengujian kanthi nggunakake 4 mmol gamma-glutamyl-3-carboxy-4-nitroanilide lan 100 mmol glycylglycine saben liter ngasilake aktivitas sing padha karo sing ditindakake kanthi nggunakake 4 mmol gamma-glutamyl-4-nitroanilide lan 40 mmol saka glycylglycine saben liter.Konsentrasi donor carboxy lan glycylglycine iki uga "biaya optimal" lan ora ana masalah prosedural nalika digunakake.
